Pu•MA System ELISA: Mouse Interleukin-6
Mouse IL-6 (Interleukin-6) is a cytokine that plays an essential role in the differentiation of B-cells into Ig-secreting cells. It acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It also induces myeloma and plasmacytoma growth, nerve cell differentiation, and acute phase reactant proteins in hepatocytes. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL11, IL-27, LIF, and OSM. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes.
The workflow for the Pu•MA System cytokine immunoassay is shown in Figure 1. All assay reagents are prepared in advance and then loaded into Pu•MA System flowchips using single or 8-channel pipettes. The wells are conveniently located on standard 384 multiwell plate spacings. The flowchips are now ready to be loaded into the Pu•MA System for “hands-off” processing. The ELISA steps are automatically performed by the system using preloaded protocols. Once the assay is complete, absorbance results are read on your Plate Reader.
Pu•MA System Complete Workflow: 2-3 hours “hands-off”
Figure 1. Schematic Pu•MA System assay workflow.
The adaption of existing ELISA kits and/or antibody pairs is straightforward with the Pu•MA System. Assay buffers optimized for the Pu•MA System flowchips are provided for sample and antibody dilutions. Blocking solutions and wash buffers are also provided. The IL-8 assays are adapted from BioLegend ELISA Max Kits (p/n 431501, 431502, & 431503). The reagents required for the assay are shown in the table.
Assay antibody reagents were prepared according to the dilutions shown in the Table using Pu•MA Assay Buffer (PAB). IL-6 standards were reconstituted according to instruction provided by BioLegend. A 1:2 serial dilution series of Mouse IL-6 Standard was prepared starting at 100 pg/well using PAB. 20 μl of each reagent was added to the appropriate wells (see Fig 3) except for the Stop Solution where 10 μl was dispensed. Four replicates were run per concentration. The flowchips were loaded into a Pu•MA System and processed using the IL-6 Assay Protocol. Plates were read within 5 minutes of being finished on an absorbance plate reader at 450 nm (Tecan Spectrafluor Plus).
|Capture Ab||Mouse IL-6 ELISA Max Capture
|Block||Pu•MA Blocking Buffer||PFI|
|Sample||Mouse IL-6 Standard||BL|
|1°Ab||Mouse IL-6 ELISA Max Detection
|Wash||Pu•MA Wash Buffer||PFI|
|Stop||Pu•MA Stop Solution||PFI|
Pu•MA System Mouse IL-6 Standard Curve
Figure 2. Response of Cytokine Standards for ELISA run on Pu•MA System. ELISA antibody pairs and standards were obtained from BioLegend. Absorbance was read at 450 nm. OD values shown are background subtracted.
How It Works
The Pu•MA Flowchip and System uses established antibody pairs to perform an automated ELISA. All assay reagents are loaded into reservoirs and then moved one at a time through the “Assay Channel” by the Pu•MA System. Preloaded protocols execute all fluid transfer and incubation steps The system incorporates patented valve-less fluidic switching (VLFS) to precisely control fluid movement in a flowchip. Use of microfluidics reduces
both incubation times and reagent volumes.
Reagent Loading Setup
- Low sample/reagent usage: < 20 μl/well)
- Enhanced kinetics: Faster Assay Results
- Effi cient fl uid removal: Less Wash Required
Figure 3. Pu•MA Flowchip reagent loading setup.
Microfluidic Assay Workflow
- Compact benchtop system
- Easy top-loading of flowchips
- Precision pneumatic control
- Touchscreen-driven interface
- Preloaded assay protocols
- Simple Select and Run operation
Reagents & Flowchips
- Validated immunoassays
- Open platform for existing Ab pairs
- Works with standard pipettors & tips