3D Cell Technology

Practical Microfluidics for Bioanalysis

The Pu·MA System

Automated 3D Cellular Assays

The Pu•MA System is a practical and affordable benchtop instrument, ideal for running physiologically complex spheroid and microtissue assays for toxicity testing and oncology discovery as well as diabetes and metabolic disease research. The system fits into standard incubators and provides gas exchange to the cell sample chambers. Complex assay protocols can be setup to automatically run from hours to days.

Protected sample chamber

Pu·MA System Benefits


Mimic in vivo conditions

Perform multi-dosing protocols and multiple media exchanges, all within your lab incubator, for extended periods.

Adapt to new assay needs

One system and flowchip accommodates a broad range of cell-based and biochemical assays.

Handle liquids gently

Spheroids and microtissues reside in a protective chamber within the well, shielding cells from flow-induced sheer forces.

Detect results easily

Clear-bottom wells enable use of imagers and point-readers for cell-based and biochemical assays.

Walk-away convenience

Protocols are fully automated, even with multiple media/reagent exchanges.

How it Works

All assay reagents are loaded into reservoirs and then moved one at a time through microfluidic channels by the Pu·MA System. Preloaded protocols execute all fluid transfer and incubation steps. The system incorporates patented valve-less fluidic switching (VLFS) to precisely control fluid movement in a flowchip. Use of microfluidics reduces both required incubation times and reagent volumes.

3D Cell Assay Workflows

Spheroids or microtissues are loaded into a “protective chamber” in sample wells

  • 32 samples can be loaded per plate

Reagents are loaded into adjacent reservoir wells

  • Up to six reagents or media
  • Load with standard single or multichannel pipettes

Pu·MA System automatically performs all reagent transfers

  • Media exchange, compound addition, etc.
  • Separate reservoir for waste media and reagents

Enables a wide range of assay readouts

  • Cell supernatant sampling
  • High content imaging
  • ATP viability
  • Metabolomic sampling
complete assay workflow

Featured News

Jul 9, 2020 Protein Fluidics' Organoid Research Award: Prof. Matthew E. Burow & team (Tulane Univ)
Jan 25, 2020
Protein Fluidics launches Pu·MA System 3D for automated organoid assays
December 4, 2018
Protein Fluidics partners with BioLegend to provide Assay Products

May 4, 2018 Protein Fluidics announces the Pu·MA system
October 1, 2016 Protein Fluidics moves into new headquarters in Burlingame, CA
April 6, 2016 Protein Fluidics closes Series seed round of investment

For Research Use Only. Not for diagnostic use. Protein Fluidics, Protein Fluidics Logo and Pu•MA System are registered trademarks of Protein Fluidics, Inc. in the United States and internationally. All rights reserved. © 2020 PROTEIN FLUIDICS, INC.