Pu·MA System

 Automated 3D Cell-Based Assays


Learn MoreContact Us

Ready to automate your 3D cell-based assay?

At Protein Fluidics we enable automated assays for 3D cell models, organoids or spheroids (with or without ECM) to achieve multifunctional profiling.  By combining microfluidics, proprietary flowchip design and 3D assay technology, we can streamline your  assay workflow. We support applications such as oncology, neuroscience, drug discovery and metabolic disease research.

Pu·MA System

The Pu·MA System with organoids within the 3D Flowchips enable the automated workflows. This system maintains the organoids in a unique sample chamber within the flowchip and allows easy media, metabolite or reagent exchanges at the touch of a button.  As a result, you can get get multiple data points from one single assay workflow using the Pu·MA System.


3D Assay Technology

Our proprietary microfluidic technology allows for precise control of fluid movement within the flowchips. The various protocol steps are executed via the touchscreen on the Pu·MA System.

Assay workflows enabled by our technology:

  • In situ supernatant sampling
  • Sequential drug addition
  • In situ lysis for metabolomics
  • Immunofluorescence workflow

Organoid Research Award (North America)

At Protein Fluidics we enable researchers to achieve their next breakthrough using automated assays. We support applications such as oncology, T-cell therapy, neuroscience, metabolism and drug-discovery.

Tell us, how would you benefit from an automated workflow for your 3D cell-models?

Apply today for our Organoid Research Award (North America).

Award: A Pu·MA System 3D for your Lab

Submission: Dates: Oct 1 – Dec 1, 2021


HeLa Spheroids stained for apoptotic and mitochondrial markers and imaged at 10X and 20X

Workflow for Multifunctional Profiling

multifunctional profiling from organoids

Featured Application

Transfer, Automated Staining, and Imaging of Organoids in Matrigel using the Pu·MA System
View our webinar recording featuring this application
HeLa Spheroids stained for apoptotic and mitochondrial markers and imaged at 10X and 20X

Featured Publication

In-depth characterization of a new patient-derived xenograft model for metaplastic breast carcinoma to identify viable biologic targets and patterns of matrix evolution within rare tumor types.

Matossian MD et al., Clinical and Translational Oncology Aug 9 2021 epub

Figure 4C from the publication showing TU-BcX-4IC organoids treated with different paclitaxel concentration and stained with live/dead cytotoxicity and Hoechst nuclear stain using the Pu·MA system. Confocal images of the organoids were acquired within the flowchips.


Check out the multifunctional profiling data from different 3D cell models in our various technical resources


Join the Protein Fluidics team at one of these upcoming webinars or conferences!


Oct 19–20

Poster Presentation @
The NYSCF Conference

Event Details 

Joint Webinar with Promega

Oct 20

Assessing Metabolic State of

3D Tumor Models

Register Today!


Oct 26–27

Talk & Poster @

SLAS2021 Building Biology in 3D

Contact Us

Protein Fluidics, Inc.

875 Cowan Road, Suite B,
Burlingame, CA 94010

+1 650 529 5080


#pumasystem  #flowchip  #3dcellassay

Our Company

For Research Use Only. Not for diagnostic use. Protein Fluidics, Protein Fluidics Logo and Pu•MA System are registered trademarks of Protein Fluidics, Inc. in the United States and internationally. All rights reserved. © 2021 PROTEIN FLUIDICS, INC.