In Situ Supernatant Sampling

One of the key steps during an assay is to analyze various secreted factors from organoids or 3D cell models. This is important for research in drug discovery, compound screening or understanding the biology of the organoids. Normally this process is very manual and labor intensive. Also, taking plates out of the incubator environment can affect the physiological response.

The Pu·MA System 3D enables automated in situ supernatant sampling as a part of the workflow within the flowchips. You can collect supernatants at multiple time points during the course of an assay without disturbing the spheroid or organoid. The conditioned media collected can then contribute to further phenotypic and physiological information about the 3D cell models being studied.

The tabs below showcase this application of in situ supernatant sampling using the  Pu·MA System.

TNBC organoids

Automated  In Situ Sampling and Metabolite Analysis from Tumoroid Samples

This schematic demonstrates the use of the Pu·MA System for timed in situ sampling along with drug treatment followed by analysis of secreted metabolites using metabolite assays from Promega.

Metabolite analysis from 3D cell models

 

 

Automated  In Situ Sampling and Metabolite Analysis from Tumoroid Samples

This tech note demonstrates automated timed in situ sampling along with drug treatment using the Pu·MA System followed by analysis of secreted metabolites with Promega assays.
You can learn how you can:

  • Collect up to 7 custom timed supernatant samples
  • 20X reduction in pipetting steps compared to manual procedure
  • Only 15-min flowchip prep time & 5-min protocol set-up time
  • >95% fluid transfer reproducibility 

 

 

Monitoring metabolite dynamics in patient-derived tumoroids using automated microfluidic Pu·MA System

We presented this data at AACR2022 on automated time-course sampling of supernatants from breast cancer tumoroids coupled with highly sensitive luminescence-based assays for important metabolites, in this case, lactate using Promega’s Lactate-Glo assay . We showed  the use of Pu·MA System for multifunctional profiling metabolite analysis of patient-derived tumoroids to different drug treatments and its use in disease modeling.

 

Multifunctional profiling of triple-negative breast cancer patient-derived tumoroids for disease modeling

Cromwell EF et al., SLAS Discovery (2022) Feb 4; S2472-5552(22)00011-9. Online ahead of print.  epub link

 

3D flowchips for Pu.MA System

 

 

 

 

 

 

 

 

 

 

 

 

Here is a clip from an event recording:

Watch full recordings:

Assessing Metabolic State of 3D Tumor Models

In this joint webinar by Protein Fluidics and Promega, scientists will showed how 3D-optimized metabolite assays can be used with cell models to assess their metabolic state. An automated Pu·MA System workflow to capture dynamic metabolite secretion from patient-derived tumoroids was featured.

Automated Sampling of Patient-Derived 3D Cell Models for Metabolite Analysis

This presentation will discuss a novel method of automated time-course sampling of supernatants from 3D cell models coupled with highly sensitive luminescence-based assays for important metabolites such as lactate and glutamate. We show the use of this system for profiling metabolism response of PD tumoroids to different treatments and its use in disease modeling.

If you have questions about using the Pu·MA System for applications with your samples, please reach out via the Contact Us page or you can Request a Quote.

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Burlingame, CA 94010

+1 650 529 5080

info@proteinfluidics.com

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For Research Use Only. Not for diagnostic use. Protein Fluidics, Protein Fluidics Logo and Pu·MA System are registered trademarks of Protein Fluidics, Inc. in the United States and internationally. All other trademarks belong to their respective owners. All rights reserved. © 2024 PROTEIN FLUIDICS, INC.