Immunofluorescence Staining for Biomarkers

Immunofluorescence Staining for Biomarkers

A key challenge when performing drug or compound testing followed by biomarker detection, is the manual and labor intensive protocol. The immunofluorescence workflow has mutliple incubation and wash steps which can be additionally complicated when working with small 3D cell models such as organoid, spheroids or tumoroids. 

Using the Pu·MA System 3D you can automate both drug treatment and the immunofluorescence workflow within the flowchips. This application can be performed with minimum perturbation to the samples which remain with the protected sample chamber,

The tabs below showcase this application using the  Pu·MA System.

Improved Assays with Magnetically Coated 3D Cell Models

PDO stained after drug treatment

This application note demonstrates the use of the Pu·MA System for three different organoids assays using different types of 3D cell models:

  • media sampling for secreted factors
  • drug treatment and staining
  • immunofluorescence (IF) staining for biomarkers

 

 

Patient-derived organoids Poster SLAS2021

Novel Assay Methods for Cancer Patient-Derived Organoids

We presented data about how you can perform supernatant sampling from individual organoids during the course of an assay. The samples of conditioned media were collected from treated and untreated organoids, and subsequently VEGF (Vascular Endothelial growth factor) secretion into the medium was measured using ELISA.

 

Immunofluorescence Workflow

Organoids, media and reagents are loaded into the flowchips. The Pu·MA System performs the automated steps described below in the workflow.

 

 

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