High throughput drug response profiling of primary colorectal tumor models using a novel automation workflow and AI-assisted image analysis

This poster was presented at MPS 2024. We demonstrated an automated workflow to dispense and assay 3D cell models using the spheroONE and Pu·MA System® EC

• Spheroids and organoids are automatically sorted, isolated and dispensed into flowchips for downstream assays providing precise control over the size and number of spheroids.
• Assays and fluid exchanges are performed in a novel microfluidic device that protects the cell models and enhances precision and control of the assay steps.
• The ability to analyze spheroids and tumoroids in situ in order to capture toxicity information and perform functional assays shows great promise for disease modeling and drug discovery.