Customer Support Portal

Tech Note1-Matrigel Grown organoids

Thank you for being a valued Protein Fluidics customer! 

On this page, you will find:

  • Videos for set-up, use & maintenance of Pu·MA System
  • Product Documents such as User Guide & Software Guide
  • App Notes, Tech Notes, Publications & Posters


If you have any questions, please feel free to email us: support@proteinfluidics.com

Recommended Best Maintenance Practices

Pu·MA System Set Up & Maintenance Videos

These short videos cover the content we had shared with you during the installation and training sessions. 
If at any point you feel you would like to revisit the training, we are happy to schedule a one-on-one Teams or Zoom session.

Pu·MA System Set-up

Dispensing in Flowchips

Placing Flowchips in Holder

Loading Flowchip Holder
into Pu·MA System

Cleaning the Vacuum Trap

https://youtu.be/IiSXkp20DZo

Routine Cleaning of the Membrane and Gaskets

Vacuum Manifold Cleaning

https://youtu.be/KDYsVcQHuWE

Recommended Best Practices

User & Software Guides

These are PDFs of documents pertaining to Pu·MA System use and maintenance  (also supplied with the system). 

Quick Guide

User’s Guide

Software Guide

Protocols & Guidelines

These are PDFs of documents pertaining to Pu·MA System Protocols and Guidelines to run assays. 

IF Staining Protocol

Pu·MA Protocols Description

How to use Pu·MA System Template

Installation & Training Documents

These are PDFs of documents you received during the installation and training session for the Pu·MA System. 

Installation Module 1

System Setup Guide

Installation Module 2

3D Flowchip Setup Guide

Installation Module 3

Organoid Assay Setup Guide

App Notes & Tech Notes

These are PDFs of app notes & technotes with data generated using the Pu·MA System. 

Metabolite analysis of 3D cell models

Technote: Automated dyanmic in sity sampling for metabolite analysis if tumoroids

Tech Note1-Matrigel Grown organoids

Technote: Transfer, automated staining & imaging of organoids in Matrigel

Technote: Automated IF staining & imaging of breast cancer spheroids

App Note: Automated assays with 3D cell models with ECM

App Note: Automated biomarker identification using IF staining followed by confocal imaging

thumbnail of app note #8

App note: Improved assays with magentically coated 3D cell models

thumbnail of app note #7

App note: Automated sequential drug addition workflow & analysis of neurospheroids

thumbnail of Appnote#6

App note: Pu·MA System 3D for Automated Organoid Assays, In Situ Sampling and In Situ Imaging

thumbnail of appnote#5

App note: Use of Pu·MA System 3D for Single Spheroid Assays with Downstream Metabolomics

Thumbnail of appnote#4

App note: Use of Pu·MA System 3 for spheroid toxicity and imaging

Publications

These are links to publication which featured the Pu·MA System. 

Multifunctional profiling of triple-negative breast cancer patient-derived tumoroids for disease modeling

Cromwell E.F. et al., SLAS Discovery (2022) S2472-5552(22)00011-9 epub

In-depth characterization of a new patient-derived xenograft model for metaplastic breast carcinoma to identify viable biologic targets and patterns of matrix evolution within rare tumor types

Matossian M.D. et al., Clinical and Translational Oncology (2022) 24(1):127-144  (Aug 2021 epub)

Disease modeling with 3D cell-based assays using a novel flowchip system and high-content imaging

Cromwell E.F. et al., SLAS Technology (2021) 26(3): 237-248

Posters Presented

These are PDFs of posters with data generated using the Pu·MA System and presented at various conferences. 

Epithelial-mesenchymal transition and adipogenic differentiation biomarker identification in patient-derived 3D cell models using a microfluidic-based system

ISSCR2022

Monitoring metabolite dynamics in patient-derived tumoroids using automated microfluidic Pu·MA System

AACR2022

SLAS2022 Poster 2

Biomarker Detection in 3D Cell Models using Pu·MA System Automated Immunofluorescence Staining

SLAS2022

SLAS2022 Poster 1

Dynamic multifunctional profiling of 3D cell models with an automated microfluidic-based assay system

SLAS2022

Contact Us

Protein Fluidics, Inc.

26219 Eden Landing Rd
Hayward, CA, 94545

+1 650 529 5080

info@proteinfluidics.com

#pumasystem  #flowchip  #3dcellassay

Our Company

For Research Use Only. Not for diagnostic use. Protein Fluidics, Protein Fluidics Logo and Pu·MA System are registered trademarks of Protein Fluidics, Inc. in the United States and internationally. All other trademarks belong to their respective owners. All rights reserved. © 2024 PROTEIN FLUIDICS, INC.