Webinar Summary

In recent years, researchers have transitioned from traditional 2D assays to more complex 3D cell models, as they are shown to recapitulate the in vivo environment and serve as a more predictive tool for drug discovery.  With this transition, there has been an increased need to automate the processing and analysis of 3D cell culture assays for a more simplified, “hands-off” workflow that yields better results.

In this webinar, we present a workflow which integrates the Protein Fluidics’ Pu·MA System for automated organoid assays followed by imaging using ImageXpress® Micro Confocal High-Content Imaging System.

Presenters

• Evan F. Cromwell, PhD (President & CEO, Protein Fluidics, Inc.)
• Oksana Sirenko, PhD (Senior Research Scientist, Molecular Devices, Inc.)

Learn how to

• Automate media exchange, sampling, and staining of complex 3D systems such as spheroids, organoids, and tumoroids
• Acquire quality images at high throughput with water immersion objectives
• Measure and analyze calcium oscillations in neuro-spheroids using the FLIPR® Penta High-Throughput Cellular Screening System
• Perform 3D volumetric analysis and other custom analyses for phenotypic characterization of compound effects