Practical Microfluidics for Bioanalysis
The PuMA System is a pneumatically driven microfluidic platform for immunoassays
The PuMA is a portable and affordable benchtop instrument that uses your existing reagents and materials. Our proprietary Valve-less Fluidic Switching (VLFS) technology reduces a typical 4-6 hour assay down to < 1 hour. Sample volumes are also reduced down to 1/10th of typical requirements. It runs off of a user-friendly interface and allows for easy data transfer with your existing plate reader instruments for data collection and analysis.
Valve-Less Fluidic Switching
Valve-Less Fluidic Switching (VLFS) is a patent pending technology that allows precise fluid control in a microfluidic device without the need of mechanical microvalves or integrated moving parts. Benefits include:
- Enables a broad range of immunoassays and other life science applications
- Programmable & automated flow switching and combinatorial mixing
- Compatible with injection molded thermoplastics for large scale production
How does it work? Microfluidic flowchips are setup as a combination of Reservoirs (A,B,C & D) and Nodes (N) connected by channels. Hydrophobic barriers are used to keep fluids in desired wells. Pneumatic pressure gradients are applied to transfer fluid from one Reservoir to another via a Node. An example is shown below where two reagents are transferred to different wells through the same Node. Watch a movie of VLFS in action!